Blood samples are preferred for DNA profile testing because the method is very sensitive to DNA quality. Analysis can be performed on buccal swab samples, but swabs must be taken properly. If DNA profile testing is unsuccessful due to DNA quality issues, we charge 50 % of full price.
Establishing a DNA profile means analyzing universal DNA regions (microsatellites) characterized by length variations. An individual carries two alleles on each tested microsatellite. These two alleles can differ in length or they can be the same. By testing sufficient number of microsatellites we can establish unique, individual specific DNA profile also named ''DNA fingerprint''. DNA profile is useful for animal identification (loss, theft, microchip failure...), parentage analysis, breeding plans and exclusion of genetic diseases (clear by parentage).
DNA profile can be generated using 2 different sets of markers:
• ISAG 2006: 22 STR markers (short tandem repeats) are tested
• ISAG 2020: 231 SNP markers (single nucleotide polymorphisms) are tested
DNA profiles generated using STR and SNP markers are not interchangeable. This means that parentage verification or identity confirmation is possible only when all animals have DNA profiles established with the same set of markers.
Quality control
Laboratory regularly participates in international quality control comparison test, which is organized every 2 years by ISAG (International Society of Animal Genetics). On latest test in 2022-2023 EVG laboratory was 100% successful and achieved the highest rank. Latest Certificate:
